Table 1.

Circularly permuted TnAK that retain in vivo phosphotransferase activity

Gene sequence (base pairs from adk)	Primary sequence (TnAK residues)	Number of occurrences
4-660 fused to 1-4	2-220 fused to 1-2	1
13-660 fused to 1-18	5-220 fused to 1-5	1
76-660 fused to 1-80	26-220 fused to 1-26	5
85-660 fused to 1-89	29-220 fused to 1-29	3
121-660 fused to 1-125	41-220 fused to 1-41	13
307-660 fused to 1-311	103-220 fused to 1-103	1
337-660 fused to 1-341	113-220 fused to 1-113	4
424-660 fused to 1-424	142-220 fused to 1-142	2
535-660 fused to 1-539	179-220 fused to 1-179	1
550-660 fused to 1-554	184-220 fused to 1-184	18
583-660 fused to 1-587	195-220 fused to 1-195	3
601-660 fused to 1-605	201-220 fused to 1-201	1
619-660 fused to 1-623	207-220 fused to 1-207	2
625-660 fused to 1-629	209-220 fused to 1-209	4
649-660 fused to 1-653	217-220 fused to 1-217	1