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. 2011 Dec 30;40(9):3886–3897. doi: 10.1093/nar/gkr1283

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Upper panel, kinetics of in vitro ϕ29 TP-DNA amplification with wild-type and mutant TPs. The amplification assays were carried out as described in ‘Materials and Methods’ section in the presence of 10 ng of DNA polymerase, 5 ng of either wild-type or the indicated mutant TP. After incubation for the indicated times at 30°C, the reaction was stopped and quantitated as total amount (in nanograms) of dNTP incorporated. Data are represented as mean ± SD corresponding to three independent experiments. In the lower panel, numbers show the amplification factor calculated as the ratio between the amount of DNA at the end of the reaction (input DNA plus synthesized DNA) and the amount of input DNA.