. 2011 Dec 30;40(9):3886–3897. doi: 10.1093/nar/gkr1283

Table 1.

Functions of wild-type and mutant ϕ29 TPs

ϕ29 TP	TP-dAMP formation^a		TP-DNA replication^a	Transition^a^,^c	TP-DNA amplification
	TP-DNA^b	No template			(%)^a Amplification factor^d
wild-type	100	100	100	1	100	50 ± 7
R158A	4 ± 1.5	n.d.	17 ± 3	n.d.	1 ± 0.2	1 ± 0.1
R169A	23 ± 6	n.d.	75 ± 13	1.7 ± 0.4	3 ± 0.3	3 ± 0.2
E191A	94 ± 11	30 ± 8	106 ± 17	2.2 ± 0.3	69 ± 2	35 ± 6
D198A	112 ± 1	102 ± 3	66 ± 8	0.3 ± 0.2	121 ± 10	61 ± 13
Y250A	74 ± 24	10 ± 2	100 ± 20	3.3 ± 0.4	130 ± 4	66 ± 10
E252A	70 ± 28	n.d.	103 ± 25	3.2 ± 0.8	52 ± 7	27 ± 7
Q253A	23 ± 5	n.d.	55 ± 12	4.7 ± 0.7	20 ± 6	10 ± 4
R256A	20 ± 8	n.d.	76 ± 19	2.6 ± 1.2	16 ± 5	10 ± 1

Data represent the mean value and the standard deviation obtained from at least three independent experiments.

^aNumbers indicate the activity of mutant TPs with respect to the wild-type TP.

^bTP-dAMP formation using ϕ29 TP-DNA as template of the reaction.

^cTransition efficiency was calculated as the ratio of the truncated products TP-(dNMP)_6-14/TP-(dNMP)_1–2 considering such quotient 1 in the case of wild-type TP. See details in the main text.

^dThe amplification factor was calculated as the ratio between the amount of DNA at the end of the reaction (input plus synthesized DNA) and the amount of input DNA.

n.d. Not detected.