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. 2012 Jan 11;40(9):4086–4096. doi: 10.1093/nar/gkr1254

Figure 2.

Figure 2.

BC1 RNA is 2′-O-methylated. (A) Primers used to detect BC1 RNA 2′-O-methylations: BMN297, BMN155 are indicated on the BC1 secondary structure. (B) Low dNTP concentration primer extension analysis using the BMN155 primer on total brain RNA from WT mice (lanes 5 and 6), on the BC1 transcript (lanes 11 and 12), and on RNA from synaptoneurosomes (lanes 13 and 14). The arrows denote 2′-O-methylations at positions G46, C47. For each sample, the second lane shows the lower dNTP concentration; stops due to low dNTP indicate the presence of a 2′-O-methylation. Sequencing reactions are shown in lanes 1–4 and 7–10. (C) Same as in (B) using the BMN297 primer; sequencing (lanes 1–4), low dNTP concentration primer extension analysis on total brain (lanes 5 and 6), on BC1 transcript (lanes 7 and 8) and on synaptosomal preparation (lanes 9 and 10). (D) Same as in (C) using total brain (lanes 5 and 6), cytoplasmic (lanes 7 and 8) and nuclear extracts (lanes 9 and 10).

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