Figure 5.

Indirect immunofluorescence staining was performed on lung sections from control (NID1^+/–; a, d, g), NID1^–/– (b, e, h), and NID2^–/– (c, f, i) mice applying antibodies raised against α–smooth muscle actin (α-sma; a–c), CD31 (d–f), and the laminin α4 chain (Lam α4; g–i). α–Smooth muscle actin and laminin α4 stainings were performed on paraffin-embedded lung sections and CD31 staining on cryosections. Secondary antibodies conjugated to Alexa 488 (green) and Alexa 594 (red) were used to detect the primary antibodies. In a–c, the nuclei were counterstained with DAPI. Scale bars = 90 µm (a–c); = 180 µm (d–i).