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. 2012 Mar 9;287(20):16158–16167. doi: 10.1074/jbc.M112.347260

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Lysine 19 in human GLYATL2 is acetylated/deacetylated in response to treatment with the deacetylase inhibitor NAM. A, recombinant hGLYATL2 was produced in E. coli and affinity-purified as outlined under “Experimental Procedures.” Mass spectrum of the KSLEKSIPESIKVY peptide (K indicates lysine at position 19 in the protein sequence) from the wild-type hGLYATL2 protein, with different levels of ionization. The peptide targeted for MS/MS analysis is marked with a star. B, fragmentation ETD tandem mass spectrum of the KSLEK*SIPESIKVY peptide from the hGLYATL2 wild-type protein; lysine 19 is not acetylated. C, recombinant hGLYATL2 was produced in the presence of 5 mm deacetylase inhibitor NAM, and ETD MS/MS analysis was carried out on the KSLEKSIPESIKVY peptide from the NAM-treated hGLYATL2. The peptide chosen for further MS/MS analysis is marked with a star. D, CID tandem mass spectrum of the peptide shows an acetylated peptide with the sequence KSLEK_AcSIPESIKVY, where K_Ac indicates an acetylated lysine at position 19 (Lys-19).