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Quantitative analysis of acetylation levels in the KSLEKSIPESIKVY peptide of hGLYATL2 by LC/MS/MS. The peptides KSLEKSIPESIKVY where lysine 19 is not acetylated elute earlier than the same peptides with modified lysine 19. The ratio of acetylated/non-acetylated lysines in the KSLEKSIPESIKVY peptide in wild-type hGLYATL2 and NAM-treated hGLYATL2 were calculated by measuring the area under the peaks.
