FIGURE 6.

Acetylation/deacetylation of hGLYATL2 regulates enzyme activity. Wild-type hGLYATL2, K19Q, and K19R mutant proteins were produced in BL21(DE3) pLysS cells in the absence/presence of 5 mm NAM, a deacetylase inhibitor. ∼1 μg of hGLYATL2 (wild type), K19Q, and K19R mutant proteins was incubated with 50 μm oleoyl-CoA, arachidonoyl-CoA, 50 mm glycine with the addition of BSA in a molar ratio of 1:5.5 BSA:acyl-CoA as outlined under “Experimental Procedures,” and the N-oleoyl glycine (A) or N-arachidonoyl glycine (B) conjugates formed were quantified using ESI-MS. The experiment was repeated four times (three times for NAM-treated K19Q and K19R mutants), and the mean ± S.D. is shown.