TABLE 1.
The calculated Km and Vmax of human GLYATL2 wild type and the K19Q and K19R mutants with N-oleoyl-CoA, N-arachidonoyl-CoA, and glycine
Km and Vmax values for N-oleoyl-CoA, N-arachidonoyl-CoA, and glycine were calculated based on two or three independent protein purifications using the Sigma Plot Enzyme Kinetics program. Data shown are the mean ± S.E. The ratio of Vmax/Km is shown. ND, not determined.
| hGLYATL2 |
N-Oleoyl-CoA (C18:1-CoA) |
N-Arachidonoyl-CoA (C20:4-CoA) |
Glycine |
|||||
|---|---|---|---|---|---|---|---|---|
| Km | Vmax | Vmax/Km | Km | Vmax | Vmax/Km | Km | Vmax/Km | |
| μm | nmol/min/mg | μm | nmol/min/mg | mm | mmol/min/mg | |||
| Wild type | 4.3 ± 0.3 | 929.1 ± 18.6 | 215.8 | 6.8 ± 1.0 | 333.1 ± 15.8 | 49.0 | 11.2 ± 2.4 | 1230.0 ± 88.0 |
| K19Q mutant | 9.6 ± 0.9 | 1102.2 ± 153.8 | 114.3 | 62.0 ± 16.7 | 390.0 ± 61.4 | 6.3 | ND | ND |
| K19R mutant | 28.7 ± 2.0 | 2037.7 ± 91.3 | 71.3 | ND | ND | ND | ND | |