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. 2012 Mar 26;287(20):16975–16986. doi: 10.1074/jbc.M111.334466

FIGURE 3.

FIGURE 3.

The role of hypoxia and HIF-1α and HIF-2α in controlling PHD2 promoter activity in NP cells. A, schematic of PHD2 reporters used for transfections (P2P-WT and P2P-MT). HRE site “CGTGCA” is muted to “ATAATA” in P2P-MT. B, although P2P-WT shows hypoxic induction in activity, the HRE mutation abolishes hypoxic induction in activity (P2P-MT). C and D, hypoxic induction of PHD2 promoter activity was suppressed by siRNA silencing of HIF-1α (C) but not HIF-2α (D). Co, Si-control. E, overexpression of HIF-1α induced PHD2 promoter activity (P2P-WT) in a dose-dependent fashion, P2P-MT activity was not affected. F, overexpression of HIF-2α did not increase the activity both P2P-WT and P2P-MT. Data represent mean ± S.E. of three independent experiments performed in triplicate (n = 3). *, p < 0.05. ns, not significant.