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. 2012 Mar 26;287(20):16975–16986. doi: 10.1074/jbc.M111.334466

FIGURE 6.

FIGURE 6.

Role of HIF-1α in controlling PHD expression. A, immunofluorescence analysis of GFP in NP cells transduced with control lentivirus expressing GFP (LV-GFP) shows high transduction efficiency. Magnification ×20. B, real-time RT-PCR analysis of PHD expression in NP cells transduced with LV-GFP or LV-CA-HIF-1α. Note that the significant induction of PHD2 and PHD3 was seen in the cells transduced by LV-CAHIF-1α, whereas PHD1 expression was not affected. HPRT, hypoxanthine phosphoribosyl transferase. C, Western blot analysis of cells transduced with LV-GFP or LV-CAHIF-1α. HIF-1α was accumulated in LV-CAHIF-1α-transduced cells compared with controls (LV-GFP). Note that the induction of PHD2 and PHD3 was seen in the cells transduced by LV-CAHIF-1α, whereas PHD1 expression was not affected. D, densitometric analysis of multiple blots from the experiment described in C above. As expected, relative HIF-1α level in LV-CAHIF-1α group was higher compared with LV-GFP. Expression of PHD2 and PHD3 was significantly increased by LV-CAHIF-1α. PHD1 expression remained unchanged. Data are represented as mean ± S.E. of three independent experiments performed in triplicate (n = 3). *, p < 0.05. ns, not significant.