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. 2012 Mar 20;287(20):16454–16466. doi: 10.1074/jbc.M112.362137

FIGURE 1.

FIGURE 1.

LRP5 and LRP6 expression in MEFs and MECs. A, lrp5 and lrp6 mRNA expression in wild type, lrp5−/−, and lrp6−/− MEFs. To test for compensatory expression of alternate LRP species in knock-out cells, lrp5 and lrp6 mRNA was quantified using qRT-PCR analysis of RNA harvested from wild type, lrp5−/−, and lrp6−/− MEFs. Values shown represent -fold changes compared with wild type MEFs (set to 1) (after normalization to housekeeping genes). B, LRP5 and LRP6 protein expression in wild type, lrp5−/−, and lrp6−/− MEFs. Western blots of protein lysates prepared from wild type, lrp5−/−, and lrp6−/− MEFs after probing with anti-LRP5 or -LRP6 (and β-actin) antibodies are shown. C, comparison of the relative signal per molecule for anti-LRP5 and anti-LRP6 antibodies. HEK293T cells were transfected with LRP5-myc and LRP6-myc plasmids, and doubling dilutions of total protein (10, 5, and 2.5 μg) were probed with anti-myc antibody and with anti-LRP5 or -LRP6 antibody to determine the relative signal with respect to the myc tag (image analysis is described under “Experimental Procedures”). D, determination of relative LRP5 and LRP6 protein expression in MEFs and MECs. Protein extracts prepared from MEFs and MECs were analyzed by SDS-PAGE, and the relative amounts of LRP5 and LRP6 expressed by the two cell types were determined. Error bars show standard deviations.