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SFN treatment alters cell cycle progression but does not induce apoptosis. A, SFN treatment causes loss of cells in G1 and accumulation of cells in G2/M. Keratinocytes were treated with 0 or 20 μm SFN for 24 or 48 h before cell sorting. Similar results were observed in each of three experiments. B, keratinocytes were treated for 24 and 48 h, and extracts were prepared for detection of the indicated cell cycle regulators. Similar results were observed in each of three experiments.
