FIGURE 6.

Analysis of products formed from [α-³²P]dATP and [α-³²P]rATP. Reaction mixtures (20 μl), as described in the legend for Fig. 1, containing 100 μm [α-³²P]dATP (611 cpm/pmol) or 200 μm [α-³²P]ATP (1090 cpm/pmol), 0.25 or 0.75 μm T. kodakaraensis DNA primase complex (lanes 2, 3, 9, and 10), and p41 subunit (lanes 4, 5, 11, and 12) or mutant (m) p41 subunit (lanes 6, 7, 13, and 14) were incubated for 30 min at 70 °C. Reactions containing 0.25 μm of the p41 subunit were incubated for 5 (lane 15), 10 (lane 16), 15 (lane 17), and 60 min (lane 18). Aliquots (1 μl) from reactions were separated by TLC on PEI plates in 0.3 m LiCl, after which plates were dried and quantified by phosphorimaging. d/rAMP der., dAMP/rAMP derivative; rAMP, 5′-riboadenosyl monophosphate.