FIGURE 5.

Analysis of c-Myc interaction with vIRF-3. A, schematic representation of c-myc deletion constructs. The shaded boxes indicate vIRF-3-binding domains. MBI and MBII, Myc conserved domains I and II; TAD, transcription activation domain; NLS, nuclear localization signal; BR, basic region; HLH, helix-loop-helix; LZ, leucine zipper. B, HA-tagged c-Myc deletion mutants were in vitro translated using the TnT T7 quick coupled transcription/translation system and incubated with vIRF-3-FL fused to GST or GST alone immobilized on glutathione-Sepharose beads. The bound proteins were eluted and resolved on 10% SDS-PAGE followed by Western blot with anti-HA antibodies. 10% of c-Myc deletion mutant protein input is shown below (10% input). aa, amino acids; N.S., nonspecific.