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. 2012 Mar 15;287(20):16488–16498. doi: 10.1074/jbc.M112.341180

FIGURE 3.

FIGURE 3.

Effects of E22O injection on growth and hemolymph ecdysteroid titer of B. mori. A, precocious pupation induced by injection of 30 μl of Sf9-E22O-conditioned medium into newly molted penultimate (4th) instar larvae. Left, a control pupa metamorphosed from an intact 5th instar larva; right, a miniature precocious pupa induced by E22O injection. 10 % of the E22O-injected larvae started spinning a week later and half of them pupated precociously. B, molting of the penultimate instar larvae after injecting 30 μl of the conditioned medium of either Sf9 (control) or Sf9-E22O cells just before the head capsule slippage (day 3.5). C, pupal development after injecting 30 μl of the conditioned medium of either Sf9 (control) or Sf9-E22O 2 days after pupation. D, pupal period of control and E22O-injected pupae. Only pupae that eclosed as adults normally were included in the calculation. Asterisks (**) indicate that the values are significantly different (p < 0.01) by Student's t test. E, ecdysteroid titers of control and E22O-injected pupae on day 4 (2 days after the medium injection). Error bars represent S.E. (n = 4).