TABLE 2.
Effects of E22O gene expression on the growth of B. mori larvae
The E22O cDNA was introduced into B. mori by in vivo lipofection at the beginning of 3rd larval instar, and larval growth was subsequently monitored.
| Plasmid | n | Average 3rd instar period (day)a | Death during 3rd instar |
Death during 4th instar |
Precocious spinning | Ecdysis into last instar | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Larval death (1–5 days after injection) | Larval death (6–10 days after injection) | Larval death (>10 days after injection) | Unsuccessful ecdysis | Larval death | Unsuccessful ecdysis | |||||
| % | % | |||||||||
| Control plasmid | 110 | 4.7 | 1 | 0 | 0 | 0 | 5 | 1 | 0 | 93 |
| pIZT-E22O | 110 | 7.1 | 2 | 6 | 19 | 6 | 25 | 2 | 7 | 34 |
a Only larvae that molted normally into the 4th instar were included in the calculation. The two values are significantly different (p < 0.01) by Student's t test.