FIGURE 3.

Defining binding sites of mAbs that map to ^9–10FNIII. A, based on unique residues in Fn from five different species and the reactivity of each mAb, potential interaction residues in ^9–10FNIII are proposed (see also supplemental Fig. S5). B, using OmpX of E. coli as an Ail homolog (62), a model of Ail binding to ⁹FNIII in a region overlapping the synergy region (51) but exposing the RGD domain is proposed. Repeats ^7–10FNIII were structurally determined by Leahy et al. (46).