FIGURE 2.

Acceptor specificity of BbAfcB D703S glycosynthase. The reaction was carried out in 100 mm MES buffer (pH 5.0) containing 40 mm FucF and a 100 mm concentration of each acceptor for 40 min at 30 °C in the presence and absence of the D703S mutant (17 μm). The acceptors used were as follows: Lac (a), 2′-FL (b), and LNT (c). The reaction products were analyzed by HPLC-CAD. The peaks of l-fucose (Fuc), acceptor, and standard sugars (Std.) are indicated. Inset in a and b are the results of the thin-layer chromatography (TLC) analysis of the reaction products. See also Table 1 and supplemental Figs. S3–S7.