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. 2012 Mar 26;287(20):16709–16719. doi: 10.1074/jbc.M111.333781

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Phylogenetic and structural difference between two subfamilies of GH29. a, the phylogenetic analysis of enzymatically characterized GH29 α-l-fucosidases. The tree was constructed using the ClustalW program with a neighbor-joining method (55). b, comparison of the catalytic sites of BiAfcB (D172A/E217A-LNFP II complex; protein in green; ligand in yellow) and TmFuc (Fuc complex; magenta; Protein Data Bank code 1ODU) (stereoview) (44). The numbers of the residues of BiAfcB and TmFuc are labeled in green and magenta, respectively. See also supplemental Figs. S11 and S12. A., Arabidopsis; H., Homo; R., Rattus; D. discoideum, Dictyostelium discoideum; C., Canis; L., Lactobacillus; B. infantis, Bifidobacterium longum subsp. infantis; B. thetaiotaomicron, Bacteroides thetaiotaomicron; D. melanogaster, Drosophila melanogaster.