FIGURE 1.

Localization of Cav-1 in the mouse retina. Cav-1 immunoreactivity (red, left panels) is predominantly localized to Müller glia and to retinal and choroidal vascular cells with weaker labeling apparent in the RPE. The α1 isoform of Na⁺/K⁺-ATPase (green, middle panels) partially colocalizes with Cav-1 in Müller glia and in the apical RPE. Loss of Cav-1 does not result in obvious changes in Na⁺/K⁺-ATPase localization (green, middle panels). DAPI staining (blue), which labels retinal cell nuclei is indicated to the left of each panel to delineate the positions of retinal layers. The blue channel was partially removed from the images by Photoshop in order to clearly observe Cav-1 and Na⁺/K⁺-ATPase localizations in retinal nuclear layers. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.