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. 2012 Mar 21;287(20):16530–16540. doi: 10.1074/jbc.M112.343079

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Effects of IQ motif deletions on the inhibition of myosin 5a S1 ATPase activity by GST-GTD. The actin-activated ATPase activities of the truncated myosin 5a S1 were measured in EGTA (open triangles) and pCa4 conditions (closed triangles). Data are the average of three independent assays. (A) MD-IQ1–6, (B) MD-IQ1/2, (C) MD-IQ1, and (D) MD. The K_d of GST-GTD to myosin 5a S1 constructs were obtained by a hyperbolic fit. The calculated K_d are 5.08 ± 1.51 μm (MD-IQ1–6), 4.33 ± 0.99 μm (MD-IQ1/2), and 3.46 ± 1.04 μm (MD-IQ1). Values are mean ± S.D. from three independent assays.