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Electrophoretic mobility shift of CaM mutants in the presence of EGTA or Ca2+ conditions. A, lanes 1 and 2, wild type CaM; lanes 3 and 4, MD-IQ1 with wild type CaM; lanes 5 and 6, CaM-B12Q; lanes 7 and 8, MD-IQ1 with CaM-B12Q. B, lanes 1 and 2, CaM-B34Q; lanes 3 and 4, MD-IQ1 with CaM-B34Q; lanes 5 and 6, CaM-B1234Q; lanes 7 and 8, MD-IQ1 with CaM-B1234Q.
