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VT1 and VT2 binding to Gb3 and adaGb3 analogues by TLC overlay. The glycolipids (1.3 μg each) were separated by TLC and detected by orcinol or by binding of either VT1 or VT2. The binding to adaGb3, OHEtadaGb3, carboxyadaGb3, urea-adaGb3, and adabisGb3 relative to Gb3 was calculated from the band intensities: VT1, 80, 20, 6, 35, and 68%; VT2, 400, 275, 140, 310, and 315%.
