FIGURE 3.

A, induction of VT1/VT2 toxicity in adaGb₃ analog-treated CHO and Jurkat cells. CHO or Jurkat cells were incubated with 0, 10, 20, or 50 μm adaGb₃ at 4 °C for 1 h. Cells were treated with 10-fold serial diluted VT1 or VT2 and incubated at 37 °C. Cell viability was monitored after 72 h and expressed as a percentage of control cells, which were treated with neither VT nor adaGb₃ analogues. B, effect of adaGb₃ analogues on CHO/Jurkat cell viability. CHO or Jurkat cells were incubated with 0, 10, 20, or 50 μm adaGb₃ (closed circles), OHEtadaGb₃ (triangles), carboxyadaGb₃ (squares), urea-adaGb₃ (diamonds), or adabisGb₃ (open circles) at 37 °C. Cell viability was monitored after 72 h and expressed as a percentage of non-treated control cells.