FIGURE 7.

Inhibition of verotoxin cytotoxicity. Increasing concentrations of adaGb₃, carboxyadaGb₃, OHEtadaGb₃, or adabisGb₃ were premixed with VT1 or VT2 dilutions at 37 °C for 1 h and then added to Vero cells for 1 h. Cells were then washed and incubated at 37 °C for 72 h. Live cells were stained with crystal violet, and viability was plotted as a percentage of untreated Vero cells. From this, the CD₅₀ values of VT1 (left)/VT2 (right) preincubated with adaGb₃ analogues were determined and plotted as a function of analog concentration.