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. 2012 May 14;7(5):e37159. doi: 10.1371/journal.pone.0037159

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Ho et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The sorted cells were stained with fluorescein-conjugated anti-CD90 and anti-GPC3 antibodies. Nuclei were counterstained by DAPI. The merge image showed the expression of CD90 and GPC3 in both cytoplasm and cell membrane.