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The sorted PLC CD90+GPC3+ cells were transfected with either 20 nM specific GPC3 siRNA or a scrambled siRNA control and incubated for 24 hours. (A) GPC3 knockdown in the target cells reduced the gene expression by 90% as measured by qRT-PCR. (B) By flow cytometry, the number of GPC3-expressing cells was decreased by 43% upon GPC3 knockdown when compared to the scrambled control (decreased from 3.9% to 2.2%).
