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. 2012 May 14;7(5):e37159. doi: 10.1371/journal.pone.0037159

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Ho et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Cell proliferation was assessed after GPC3 knockdown in PLC CD90⁺GPC3⁺ cancer stem cells. No significant effect of GPC3 on liver cancer stem cell proliferation was found. (B) Knockdown of GPC3 in PLC CD90⁺GPC3⁺ cancer stem cells by siRNA did not affect their colony formation ability, indicating that GPC3 had no impact on clonogenicity of the liver cancer stem cells.