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. 2012 May 14;7(5):e37142. doi: 10.1371/journal.pone.0037142

Figure 1. Test for the presence of mitochondrial and nuclear DNA in serial dilution series' T-DNA I & T-DNA II.

Figure 1

T-DNA I was used as template in A–C and T-DNA II in D–F. The highest dilution step in which the presence of nuclear DNA was revealed using both nuclear and numt-specific primers was step 3 (10−3, B, C) and step 4 (10−4) for mtDNA primers (A) on T-DNA I. For T-DNA II, dilution step 1 (100) was the highest dilution showing residual nuclear and numt amplification and step 5 (10−4) for mtDNA. Controls (ctrl) are no template controls in A–D and positive controls in E–F. M: DNA ladder (100 bp).