Table 1. Primers used for PCR.
| Amplified fragments | Primer name | Primer sequence (5′>3′) |
|---|---|---|
| TGFBI-WT | TGFBI-WT-F | GTCACTCGAGTCGGTCGCTAGCTCGCTC |
| TGFBI-WT-R | GTCAAAGCTTCTATGCTTCATCCTCTCTAATAAC | |
| TGFBI-T538P-I | TGFBI-WT-F | GTCACTCGAGTCGGTCGCTAGCTCGCTC |
| TGFBI-538-R | CAAAGACTGG*GTAGACTCCT | |
| TGFBI-R555W-I | TGFBI-WT-F | GTCACTCGAGTCGGTCGCTAGCTCGCTC |
| TGFBI-555-R | AGTCTGCTCCA*TTCTCTTGG | |
| TGFBI-T538P-II | TGFBI-538-F | AGGAGTCTACC*CAGTCTTTG |
| TGFBI-WT-R | GTCAAAGCTTCTATGCTTCATCCTCTCTAATAAC | |
| TGFBI-R555W-II | TGFBI-555-F | CCAAGAGAAT*GGAGCAGACT |
| TGFBI-WT-R | GTCAAAGCTTCTATGCTTCATCCTCTCTAATAAC | |
| TGFBI | TGFBI-qPCR-F | AGGACTGACGGAGACCCTCAAC |
| TGFBI-qPCR-R | TCCGCTAACCAGGATTTCATCAC | |
| GAPDH | GAPDH-F | CAGGGCTGCTTTTAACTCTGG |
| GAPDH-R | TGGGTGGAATCATATTGGAACA |
*: site of introduced mutation.