Figure 4. MSU crystals inhibit protein synthesis at concentrations that induce processing and release of IL-1ß.

WT BMDM were primed with LPS for 4 h prior to exposure MSU or MPU at indicated concentrations. A) BMDM in triplicate wells were pulse-labeled in medium containing [³H]-leucine for 15 min prior to harvest at the indicated times and the amount of [³H]-leucine incorporation was measured. B) Cells were harvested 4 h after addition of indicated concentrations of MSU and MPU. Cell lysates (cell) and culture medium (medium) were examined by immunoblotting. P38 MAPK was loading control.