Figure 2. Edin does not affect the ability of S2 cells to bind microbes.

(A–B) The effect of edin RNAi on the binding of E. coli and S. aureus in Drosophila S2 cells. Drosophila S2 cells were soaked for three days in dsRNAs and thereafter exposed to bacteria at +4°C. GFP dsRNA was used as a negative and eater dsRNA as a positive control. (C–D) The effect of edin overexpression on the binding of E. coli and S. aureus. S2 cells were transiently transfected with a pMT construct expressing edin and endogenous edin expression was knocked down with dsRNA treatments. The ability of S2 cells to bind heat-killed E. coli (A, C) or S. aureus (B, D) was measured using flow cytometry.