Figure 3. The effect of Edin on microbial binding.

500 µl of Edin-V5 containing medium were incubated with 1 ml of a bacterial suspension of live E. coli (E.c.), Serratia marcescens (S.m), Staphylococcus epidermidis (S.e.), Enterococcus faecalis (E.f.), Listeria monocytogenes (L.m.), Micrococcus luteus (M.l.), Saccharomyces cerevisiae (S.c.) or S. aureus (S.a) for 1 h with mild agitation at +4°C. Latex beads treated with BSA were used as a control. The samples were then centrifuged and the pellet was washed. Edin bound to microbes was detached by adding 20 µl of SDS-PAGE loading buffer, boiled for 10 minutes, electrophoresed on SDS-PAGE and detected using a V5 antibody. The first lane of each blot is a control sample containing 20 µl of Edin-V5 medium. The following lanes contain 30 µl of the medium incubated with the indicated microbe.