Figure 5. The effect of Edin on AMP production in vivo.

Edin RNAi and overexpression flies (edin,Rel^E20) were crossed with C564-GAL4 flies or w¹¹¹⁸ flies as a control, their offspring was infected with E. cloacae, total RNAs were extracted at indicated time points and qRT-PCR for the indicated genes was performed. (A) Expression of edin is knocked down in edin RNAi flies crossed to C564-GAL4 driver flies. (B) Edin overexpression flies express edin at a physiological level. Edin overexpression flies crossed with C564-GAL4 have slightly higher levels of edin compared to flies crossed with w¹¹¹⁸. For (A–B) the data are pooled from 2 independent experiments, and n = 8 for each sample at each time point. (C–H) The effect of edin RNAi and overexpression on the production of Diptericin B (C), Cecropin A1 (D), Attacin B (E), Attacin A (F), Attacin C (G) and Drosocin (H). n = 4 for each sample at each time point. Error bars represent the standard deviation of each sample.