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. 2012 May 14;7(5):e36520. doi: 10.1371/journal.pone.0036520

Figure 2. Decoy Wnt receptor sLRP6E1E2 reduces cytosolic β-catenin and T-cell factor transcriptional activity.

Figure 2

(a) TCF/LEF luciferase reporter assay in A549 cells. To characterize the sLRP6E1E2 effects on the Wnt3a/β-catenin signaling, cells were transfected with TOPflash (containing wild-type TCF binding sites) or FOPflash (containing mutated TCF binding sites) luciferase vector. *P<0.05 versus dE1-k35/LacZ-transduced or PBS-treated cells. (b) TCF/LEF luciferase reporter assay in H460 and H322 cells. *P<0.05 versus PBS or dE1-k35/LacZ-transduced cells with or without Wnt3a. (c) H322 cells were transduced with dE1-k35/LacZ or dE1-k35/sLRP6E1E2 (50 MOI) with or without Wnt3a. Cells were labeled with anti-β-catenin. Original magnification, ×630. (d) Semi-quantitative analysis of panel (c) results using MetaMorph® imaging analysis software. Each data point indicates mean ± SEM (each group, n = 5). **P<0.001 versus PBS or dE1-k35/LacZ-transduced cells with Wnt3a.