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. 2012 May 14;7(5):e36520. doi: 10.1371/journal.pone.0036520

Figure 4. Decoy Wnt receptor sLRP6E1E2 induces apoptosis in human lung cancer cells.

Figure 4

(a) Cells were transduced with dE1-k35/LacZ or dE1-k35/sLRP6E1E2 at (20 MOI), and photographs were taken at 72 hr later. Original magnification, ×200. (b) Detection of sLRP6E1E2-induced apoptosis by TUNEL staining. Original magnification, ×400. (c) Total number of TUNEL-positive cells per fields (mean ± SEM). *P<0.05 versus PBS or dE1-k35/LacZ treated with Wnt3a; **P<0.001 versus PBS-treated or dE1-k35/LacZ-transduced controls. n.s.  =  not significant. (d) Western analysis of sLRP6E1E2-mediated apoptosis. H460 cells were transduced with dE1-k35/LacZ or dE1-k35/sLRP6E1E2 (20 MOI). The western blot using specific antibodies against uncleaved PARP, cleaved PARP, pro-caspase-3, cleaved caspase-3, and cytochrome c. (e) H460 cells were treated as indicated above (Fig. 4d). Subcellular localization of cytochrome c was determined by western blot analysis of cytosolic and microsomal fractions.