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. 2012 May 14;7(5):e36739. doi: 10.1371/journal.pone.0036739

Figure 1. Radiation-induced phosphorylation of c-Jun and ERK1/2 in BV2 cells.

Figure 1

(A) BV2 cells were irradiated with a single dose of 10 Gy, and sampled at the time points indicated. Cell lysates were sequentially immunoblotted with indicated antibodies in Western blot assays to detect phosphorylated and total proteins of c-Jun, ERK1/2, and JNK. β-actin was used as a loading control. Increased phosphorylation was found for c-Jun and ERK1/2 post irradiation, but not for JNKs. There was no obvious change for total c-Jun. The right panel shows the fold change for phosphorylation of c-Jun (Ser63 and Ser73) and ERK1/2 following irradiation. Data represent mean ± SD and the significant differences compared to control were indicated as *p<0.05 or **p<0.01. (B) BV2 cells were irradiated with different doses as shown and phosphorylation of c-Jun and ERK1/2 was detected at 0.5 and 1 h post-irradiation. Regardless of dose levels, radiation markedly stimulated phosphorylation of c-Jun and ERK1/2.