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. 2012 May 14;7(5):e36855. doi: 10.1371/journal.pone.0036855

Figure 2. mNanog, ventx1/2, and msx1 cause distinct effects on early patterning gene expression.

Figure 2

For gain-of-function experiments, NF3-embryos were injected radially in all blastomeres with water, msx1 mRNA (0.3 ng/blastomere, red), mNanog mRNA (0.15 ng/blastomere, blue) or ventx1/2 mRNAs (0.5 ng/blastomere, green); For loss-of-function experiments, NF-2 embryos were injected twice radially in both blastomeres with control MO (30 ng/blastomere), or a 1∶1 mix of ventx1/2 MOs (30 ng/blastomere, purple). All embryos were collected at stage NF10.5 and processed for RT-QPCRs. Ectodermal, mesodermal and endodermal markers were assayed (each quantification was performed at least 3 times independently). For all RT-QPCR, graphs represent means of the fold-change calculated versus the appropriate control (fldx injected embryos in cases of overexpression and control MO for ventx1/2 knock-down) +/− s.e.m, and significance was assessed using paired t-test (*p≤0.05, **p≤0.005, ***p≤0.0005).