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. 2012 May 14;7(5):e36855. doi: 10.1371/journal.pone.0036855

Figure 3. ventx1/2 overexpression prevents multiple lineage commitment.

Figure 3

(A) NF2-embryos were injected twice in one blastomere, either with msx1 mRNAs (0.6 ng/blastomere), ventx1/2 mRNAs (1 ng/blastomere), mNanog mRNA (0,3 ng/blastomere), or with water for control; fldx was used as a lineage tracer. WISH with a k81a1 probe were performed at stage NF10.5 (left panels, animal views, dorsal side to the top). The progeny of the injected blastomere was revealed by fluorescence; white arrows indicate the injected side (right panels). (B) Sixteen-cell stage embryos (NF5) were injected in one AB4 blastomere with msx1 mRNA (0.15 ng), ventx1/2 mRNAs (0.5 ng), mNanog mRNA (0.15 ng), or water, collected at stage NF10.5 and processed for WISH with a k81a1 probe (animal views). Black stripped lines mark the border between injected and uninjected domains. (C) NF2-embryos were injected twice in one blastomere with msx1 mRNA (5 ng/blastomere), ventx1/2 mRNAs (5 ng/blastomere), ventx1/2+msx1 mRNAs (5 ng +5 ng/blastomere), or with water. WISH with a myf5 probe were performed at stage NF10.5; black arrowheads point to myf5-expressing territories (left panels, ventral views, dorsal side to the top). The progeny of the injected blastomere was revealed by fldx fluorescence; white arrows point to the injected side (right panels).