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. 2012 May 14;7(5):e36963. doi: 10.1371/journal.pone.0036963

Figure 4. Tandem arrangement of NicCL3.

Figure 4

Southern blot hybridisation of genomic DNA from (TH) synthetic tobacco Th37-5, (TO) N. tomentosiformis ac. NIC 479/84 and (TA) N. tabacum ac. SR1 digested with SpeI, BamHI and HaeII (a methylation sensitive isoschizomer of BamHI) and probed with NicCL3. Size indicators on the left are in kb. Digestion with BamHI and SpeI results in a ladder like pattern, typical of tandem repeats. Digestion is inhibited when using HaeII, indicating extensive CG methylation of tandem units.