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. Author manuscript; available in PMC: 2013 May 11.
Published in final edited form as: Mol Cell. 2012 Apr 17;46(3):299–310. doi: 10.1016/j.molcel.2012.03.019

Figure 1. Recombinant DCL-2 processes pri-milR-1 into pre-milR-1 in vitro.

Figure 1

(A) A model of the milR-1 biogenesis pathway showing that the production of milR-1 requires Dicer, Argonaute protein QDE-2, and the putative exonuclease QIP.

(B) The Coomassie blue stained SDS-PAGE gel showing the purified His-DCL-2 from Sf9 cells.

(C) Dicer assay using radiolabeled dsRNA as the substrate.

(D) Dicer assay using radiolabeled pri-milR-1 as the substrate.