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. Author manuscript; available in PMC: 2013 May 11.
Published in final edited form as: Mol Cell. 2012 Apr 17;46(3):299–310. doi: 10.1016/j.molcel.2012.03.019

Figure 4. The exosome complex is required for milR-1 maturation and is responsible for the generation of the single-stranded pre-milR-1 ladder in the qip mutants.

Figure 4

(A) Northern blot analysis of small RNA samples separated in denaturing gels showing the levels of milR-1 pre-milRNAs and milRNAs in the indicated strains.

(B) Representative small RNA species with untemplated adenosines identified by Solexa deep sequencing analysis.

(C) & (D) Northern blot analysis of small RNA samples separated in denaturing gels showing the levels of milR-1 pre-milRNAs and milRNAs in the indicated strains. dsfrh and dsrrp44 are the dsRNA knockdown strains in which frh or rrp44 dsRNA expression is induced by QA.

(E) Northern blot analysis of small RNA samples separated in native gels showing the levels of milR-1 pre-milRNAs and milRNAs in the indicated strains. “D” indicates that RNA samples were denatured by boiling. “N” represents native RNA samples.