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. Author manuscript; available in PMC: 2013 May 11.
Published in final edited form as: Mol Cell. 2012 Apr 17;46(3):299–310. doi: 10.1016/j.molcel.2012.03.019

Figure 5. QDE-2 interacts with an exosome component and promotes the trimming of single-stranded pre-milR-1 by the exosome.

Figure 5

(A) Western blot analysis showing the result of immunoprecipitation with the anti-FRH antibody in the Myc-QDE-2 strain. Cell extract was incubated with/without RNase A before immunoprecipitation. Immunoprecipitation with the FRH pre-immune (PI) serum was used as the negative control.

(B) The Coomassie blue stained SDS-PAGE gel showing purified MBP-QDE-2 from E. coli, and the silver stained SDS-PAGE gel showing purified core exosome complex from S. cerevisiae.

(C) siRNA-initiated RISC assays performed with human Ago2 (hAgo) or MBP-QDE-2.

(D) 5’ [32P] radiolabeled single-stranded pre-milR-1 incubated with the indicated purified proteins. The products were separated by a denaturing gel.