Fig. 3.

PKA-mediated cMyBP-C phosphorylation in vitro and in vivo. a 2D-gel analysis of cMYBP-C from IDCM myocardium before and after PKA treatment, showing an shift towards lower pI species, indicating increased phosphorylation. b Quantification of the changes in phosphorylation after PKA treatment. c, d Effect of high and low dose Dob administration (2 and 10 μg/kg/min; Dob2 and Dob10) on the phosphorylation of the PKA-specific Ser23/24 cTnI site (n = 5) and Ser282 cMyBP-C site (n = 6) in sham and post-MI remodeled myocardium from pigs. cTnI phosphorylation increased significantly in sham animals but this increase was attenuated in MI animals. cMyBP-C was not different between MI and sham animals. Figure adapted from Boontje et al. (2011) with permission from Elsevier. *P < 0.05, effect of Dob in a 1-way ANOVA