Fig. 3.

Transcript level for the genes encoding AlaAT homologues in shoots of wheat seedlings subjected to hypoxia. Quantitive Real Time PCR was performed on cDNA synthesized on the basis of total RNA extracted from shoots of control seedlings i.e. grown under optimal conditions and harvested 24, 48, 72, 96 and 120 h after induction of hypoxia. Development of hypoxia has been monitored by quantification of the response of gene encoding alcohol dehydrogenase (ADH1). Relative mRNA concentration is expressed in arbitrary units corresponding to the ratio of cDNA copy number of studied gene/18S rRNAcDNA copy number and presented as % of the relative mRNA concentration in control seedlings i.e. grown under optimal conditions. Results are the mean of three replicates ± SD