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. 2012 Feb 12;31(6):1105–1117. doi: 10.1007/s00299-012-1231-2

Fig. 8.

Fig. 8

Transcript level for the genes encoding AlaAT homologues in shoots of wheat subjected to nitrogen deprivation. Quantitive Real Time PCR was performed on cDNA synthesized on the basis of total RNA extracted from shoots of control seedlings and seedlings harvested 24, 48, 72, 96 and 120 h after transfer to a nitrogen-free medium. Transcription level of nitrate reductase (NR) as a marker gene for nitrogen starvation was investigated. Results are the mean of three replicates ± SD