Table 2.
The variables included into the RF analyses
| Number | Variable |
|---|---|
| 1 | Hemoglobin (g/l) |
| 2 | Hematocrit |
| 3 | Erythrocytes (×1012/l) |
| 4 | Leucocytes (×109/l) |
| 5 | MCV (fl) |
| 6 | Thrombocytes (×109/l) |
| 7 | Total serum cholesterol (mmol/l)a |
| 8 | LDL-cholesterol (mmol/l)b |
| 9 | HDL-cholesterol (mmol/l)c |
| 10 | Serum TGs (mmol/l)a |
| 11 | Creatinine (μmol/l)d |
| 12 | ASAT (U/l)d |
| 13 | ALAT (U/l)d |
| 14 | B-glucose (mmol/l) |
| 15 | γ-GT (U/l)d |
| 16 | Myristic acid, 14:0 (%)e |
| 17 | Palmitic acid, 16:0 (%)e |
| 18 | Palmitoleic acid, 16:1 (%)e |
| 19 | Stearic acid, 18:0 (%)e |
| 20 | Oleic acid, 18:1 (%)e |
| 21 | Linoleic acid, 18:2 (%)e |
| 22 | α-linolenic acid, 18:3ω-3 (%)e |
| 23 | Arachidonic acid, 20:4ω-6 (%)e |
| 24 | Eicosatetraenoic acid, 20:4ω-3 (%)e |
| 25 | Eicosapentaenoic acid, 20:5 (%)e |
| 26 | γ-linolenic acid, 18:3ω-6 (%)e |
| 27 | Docosahexaenoic acid, 22:6 (%)e |
| 28 | Docosapentaenoic acid, 22:5 (%)e |
| 29 | Behenic acid, 22:0 (%)e |
| 30 | Lignoceric acid, 24:0 (%)e |
| 31 | Nervonic acid, 24:1 (%)e |
| 32 | Retinol (μg)f |
| 33 | Carotene (μg)f |
| 34 | Vitamin D (μg)f |
| 35 | Vitamin E (mg)f |
| 36 | Thiamine B1 (mg)f |
| 37 | Riboflavin B2 (mg)f |
| 38 | Niacin B3 (mg)f |
| 39 | Pyridoxine B6 (mg)f |
| 40 | Cobalamin B12 (μg)f |
| 41 | Folate B9 (μg)f |
| 42 | Pantothenic acid B5 (mg)f |
| 43 | Biotin B7 (μg)f |
| 44 | Vitamin C (mg)f |
| 45 | Calcium (mg)f |
| 46 | Magnesium (mg)f |
| 47 | Iron (mg)f |
| 48 | Selenium (μg)f |
| 49 | Mercury (μg)f |
| 50 | Flavonoids (mg)f |
| 51 | Quercetin (mg)f |
| 52 | Myricetin (mg)f |
| 53 | Kaempferol (mg)f |
| 54 | Alcohol (E%)f |
| 55 | Total serum cholesterol (mmol/l) |
| 56 | Esterified cholesterol (mmol/l) |
| 57 | Free cholesterol (mmol/l) |
| 58 | Total FAs (mmol/l) |
| 59 | 18:2 (%) |
| 60 | Other PUFAs than 18:2 (mmol/l) |
| 61 | All PUFAs (mmol/l) |
| 62 | 22:6 (%) |
| 63 | Total TG (mmol/l) |
| 64 | Total PG (mmol/l) |
| 65 | Phosphatidylcholine (mmol/l) |
| 66 | Sphingomyelin (mmol/l) |
| 67 | Total choline phospholipids (mmol/l) |
| 68 | ω-3 FAs (%) |
| 69 | ω-6 and ω-7 FAs (%) |
| 70 | ω-9 and saturated FAs (%) |
| 71 | Ratio of CH2 signal area to FA signal area |
| 72 | Ratio of TG to PG |
| 73 | Ratio of CH2 signal area to DB signal area |
| 74 | Ratio of DB signal area to FA signal area |
| 75 | Ratio of PUFAs to DB signal area |
| 76 | Ratio of PUFAs to FA |
| 77 | Average FA chain length |
The variables 1–54 were determined previously (Mursu et al. 2004) whereas the variables 55–77 were obtained from 1H NMR spectrum and were scaled so that the total serum cholesterol values determined both in the previous study (variable 7) and by the 1H NMR method (variable 55) are identical. The baseline and end-point values (VARBASE+END) or the change (VARCHANGE) values of these variables were used depending on the RF analysis
ALAT alanine transaminase, ASAT aspartate transaminase, DB double bond, FA fatty acid, MCV mean cell volume, PG phosphoglyceride, PUFA polyunsaturated fatty acid, TG triglyceride, γ-GT, gamma-glutamyl transferase
aDetermined using an enzymatic colorimetric test
bDetermined by a direct cholesterol measurement (Konelab)
cMeasured from the supernatant after magnesium chloride dextran sulphate precipitation
dMeasured using a Clinical Chemistry Analyzer (Konelab)
eSerum fatty acids were extracted using chloroform–methanol and methylated with sulphuric acid–methanol. The methylated fatty acids were analyzed by a gas chromatograph
fBased on the 4 day food record that was collected before the intervention and during the last week of the supplementation period