Table 3.

Spacer length between the two-point anchored LBT and target proteins, and the distance between the Cα atoms of N-terminus residue of the target and the anchoring residue disulfide bond

	Anchoring point	Cα atom distance (Å)	Minimal spacer length
GB1	M1-E19C	6.1	3a
p62 PB1 domain	S3-C26	6.0	3b
FKBP12	V2-T75C	5.6	3c
Grb2 SH2 domain	W60-M73C	9.9	4d

^aSaio et al. (2009)

^bSaio et al. (2010)

^cPresent study

^dSaio et al. (2011)