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. 2012 Mar 29;9:26. doi: 10.1186/1742-4690-9-26

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Copyright ©2012 Simonis et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gag induces proteasomal degradation of TRAF2. (A) Western blot of HEK293T cell extracts transfected with expressing vectors for Flag-TRAF2, HTLV-2Gag-GFP and Myc-ubiquitin. Cell extracts were immunoblotted with anti-Flag, anti-Myc, anti-GFP and anti-actin antibodies. (B) Western blot of HEK293T cells transfected with expressing vectors for Flag-TRAF2 and HTLV-2Gag-GFP, pre-treated or not with the proteasomal inhibitor MG-132 (1 μM) for 24 H. Cell extracts were immunoblotted with anti-Flag or anti-actin antibodies.